2007 Live-cell Course Info       Home • '07 Brochure • Apply • Prof. Pawley
Images and Movies
from the Course		 Sponsored By
Overview

 • UBC Brain Research Centre
    Max Cynader, Director
 • UBC Dept of Anesthesiology,     Pharmacology & Therapeutics
 • UBC Dept of Cellular and     Physiological Sciences

Lectures Draft
Images

Click the images to view the time-lapse movies
in QuickTime format.
Get QuickTime Download QuickTime player here
Movies
Faculty        
Companies
Represented
 Arabidopsis Root
Course Alumni

Movie size: 393 kb
Scanned using a Bio-Rad 2-Photon system
Courtesy of Nick White,
Oxford University
Future Course  
Brochure
APPLY
3-D Image
Processing Info
 
 White Blood Cells (Eosinophilis)
Overview

Movie size: 362 kb
White blood cells attacking infecting nematode in Rat Gut
Scanned using a Bio-Rad 2-Photon system
Courtesy of Rob Eversole,
Western Michigan University
Program
 
British Columbia
Tourist Info
 
General Info
 
Tourism    Cultured Tobacco Cells      
UBC Info

Movie Size: 412 kb
Imaged with simultaneous fluorescence and reflection imaging on a Bio-Rad MRC-1024 confocal microscope. The reflected surface image is cut away to reveal vacuoule membranes labeled with FM-143. Depth is approximately 23 µm, 77 slices. Collected by Dr. Birgit Neubohn, 198, images processed with Voxblast on a Mac 7300/200
Courtesy of Glen MacDonald,
Microscopy and Imaging Facility, University of Washington Core for Communication Research

  
   
     
                   
   Confocal of Spirogyra (1)      
  Movie size:
20X/.75 Nikon objective, 4X zoom. 0.3 um/pixel, 4 usec dwell time (Normal Scan).
60 timepoints collected 10 sec apart.
Fluor 488 nm excitation, left panel is Transmitted Light Detector, right panel is chlorophyll autofluorescence 660-700 nm. Notice pronounced focus drift in the right panel.
 
     
                   
   Confocal of Spirogyra (2)      
 

Movie size:
Same sampling parameters as 1 but showing the junction of two cells.
Notice fluorescence fading after the first few scans.

 
     
                   
   Confocal of Spirogyra (3)      

Movie size:
Same as 2, but better displays organellar motions.
 
 Confocal of Spirogyra (4)
Same field as "2" above, 80 degree rotation, 320-40 deg. of chlorophyll fluorescence. 18 µm thickness/.3 µm interval.
 
 Sasha's 2002 Cholera Toxin
Movie size: 2.01 MB
           

3D Microscopy of Living Cells Course
Email the webmaster for any questions regarding this website