"If it's not diffraction, it's statistics."

Insurgent course motto:
"It's not just diffraction, it's not just statistics,
it's BIOLOGY!"

The quality of a confocal image is limited by the number of photons recorded in each pixel. In the 4 images above, the 0.35 µm spacing can only be seen in the lower right panel where the brightest pixels have at least 16 photons/pixel.